Qualitative evaluation of early pregnancy using Rapid detection milk kit in Graded Murrah buffalo cows

“Heat-aid kit” is a rapid detection kit which was developed by the application of ELISA. This qualitative test was indicated by colour change i.e based on the presence of progesterone. The present research was carried out to evaluate the accuracy of heat aid kit, a rapid detection test for early pregnancy diagnosis in graded Murrah buffaloes. For this, the milk samples were collected from 16 buffaloes early in the morning on day 21 post insemination and were subjected to pregnancy diagnosis using this test. Development of dark/strong pink was considered as estrus and these animals were certainly non pregnant. If the colour was very pale/colourless, that the animal may be pregnant or in mid cycle and confirmation of pregnancy was done by rectal palpation on days 45-60 post insemination. It was concluded that, this test was 85.71 accurate in diagnosing pregnancy and 100 per cent in diagnosing non-pregnancy. The overall diagnostic accuracy, positive and negative predictive values of this test were 93.75, 100 and 90 per cent, respectively.